3/16/2023 0 Comments Daa converter for windows![]() ![]() For some types of errors, msConvert is able to return an error message that indicates the nature of the fault. Garbles may also occur as a file is transmitted from computer to computer. This is generally true of “mis-injections”, which produce raw file stubs. In some cases, raw data may have become garbled. The run time of conversion, however, may be lengthened by some of the available filters, particularly calculation-heavy options. Frequently these conversions can be conducted in less than one minute for each LC-MS/MS experiment. In most cases, file transcoding is a relatively rapid step. Keeps only spectra with scan of the selected polarity. Keeps only spectra with the indicated mass analyzer type. Keeps only spectra whose precursors have the specified activation type. Predicts MSn spectrum precursors to be singly or multiply charged depending on the ratio of intensity above and below the precursor m/z. Noise peak removal for spectra with precursor ions.ĭeisotopes MS level 2 spectra using Markey method.įilters ETD MSn spectrum data points, removing unreacted precursors, charge-reduced precursors, and neutral losses. Contains a wider array of options than the “threshold” filter. Keeps data whose values meet various threshold criteria. Keeps only spectra with peak counts within a given range.ĭeals with zero values in spectra - either removing them, or adding them where they are missing. Retains spectra with specific precursor m/z values. Keeps mz/intensity pairs whose m/z values fall within the specified range. Details for the different criteria can be found in the GUI tooltips. Rejects ion trap data spectra with MS level 1.Īdd or replace a spectra’s TIC/BPI metadata, usually after peak picking where the change from profile to centroided data may make the TIC and BPI values inconsistent with the revised scan data.Īdds or replaces spectrum titles according to format string given. Reorders spectra, sorting them by ascending scan start time. Selects spectra within a given time range. If used with other filters this must be set first. Performs centroiding on spectra with the selected MS levels. Recalculates the precursor m/z and charge for MS2 spectra based on the MS1 data. Use 0 to include spectra with no charge state at all. Keeps spectra that match the listed charge state. Selects only spectra with the indicated MS levels. Selects spectra by index - an index value 0-based numerical order in which the spectrum appears in the input. Basic Protocol 2 is intended to assist researchers who need to convey their data to search engines requiring simpler text formats. Both protocols address the process of conversion from instrument vendor-specific raw data formats ( Table 1) to mzML ( Martens et al., 2011), mzXML ( Pedrioli et al., 2004), mz5 ( Wilhelm et al., 2012), or MGF ( ) format. Basic Protocol 1 is intended for first-time users of ProteoWizard who feel most comfortable with graphical user interfaces, while Alternate Protocol 1 will assist researchers who are comfortable in a command-line environment. ![]() ![]() The three protocols included here are intended to complement each other. ![]() It is distinctive for its support of instruments from many instrument vendors ( Chambers et al., 2012). The ProteoWizard library and tools are designed to extract data from these proprietary formats for export in community standard formats or for direct access via its API ( Kessner et al., 2008). Tandem mass spectrometry data sets are captured to binary files or databases by the software controlling the instruments. ![]()
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